Primers Annealing Temperature Calculator What Will Be The Optimal PCR Conditions For An 88 Mer Oligo?
What will be the optimal PCR conditions for an 88 mer oligo? - primers annealing temperature calculator
The Tm of the primers are 60.5 and 76.6 degrees Celsius. What is the temperature of annealing.
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1 comments:
The melting temperatures of the 2-primers are very different, so in this case we choose the lower T, eg 60.5 C. It would be better for the TM primers change were closer.
I assume this means that the total length 88 NT-ware, or you have heard to mean the initiators? It is not clear.
As provided whatsoever, you have a product so little, I propose the following:
95 ° C for 5 min
30 cycles:
94 C for 30 seconds, 58 C for 15-30 seconds, 72 C for 15 to 30 sec
72 C 8 minutes
We hope you find it useful.
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